Journal: iScience

Article Title: Dietary fiber reduces mortality from secondary sepsis in a murine model of Clostridioides difficile infection

doi: 10.1016/j.isci.2026.115258

Figure Lengend Snippet: Plasma markers of systemic inflammation and kidney injury in CDI mice across diets (A) PCoA of Canberra distances of mean-normalized sepsis/immune marker concentrations. Points are colored by diet, and shapes indicate whether mice were humanely euthanized due to clinical sickness or survived until the experimental endpoint. Vectors show the correlation of each measured factor with PC1 and PC2, with the vector length indicating the relative strength of the correlation. Only statistically significant vectors are shown (multiple regression with Benjamini-Hochberg multiple test correction, p.adj. < 0.05). (B) Plasma concentration of each marker that significantly differed between diets (blood urea nitrogen (BUN), and immune factors sCD14, CXCL1, IL-10, IL-1B, IL-6, and TNF-a) at sacrifice. Pairwise comparisons of concentrations between diets were calculated using Kruskal-Wallis and Dunn’s post hoc tests, with p -value corrections conducted via Benjamini and Hochberg. Boxplot lines (from top to bottom) depict the 75 th , 50 th (median), and 25 th percentiles, with lines extending from the top/bottom of the boxplot indicating the largest/smallest observation within ±1.5∗IQR (inter-quartile range). P-value significance (∗∗∗∗: p < 0.0001, ∗∗∗: p < 0.001, ∗∗: p < 0.01, and ∗: p < 0.05).

Article Snippet: Soluble CD14 (sCD14) levels were quantified using the Quantikine Mouse sCD14 ELISA Kit (Catalog No. MC140, R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions.

Techniques: Clinical Proteomics, Marker, Plasmid Preparation, Concentration Assay

Journal: Scientific Reports

Article Title: Development of a newly immunoassay specific for mouse presepsin (sCD14-ST)

doi: 10.1038/s41598-022-22096-1

Figure Lengend Snippet: Specificity of the established polyclonal antibodies (N-pep2 and C-pep8) for mouse presepsin as assessed by immobilized antigens. ( A ) Sequential diluted polyclonal antibodies (culture supernatant) were incubated with immobilized recombinant mouse presepsin-Fc (sCD14-ST-Fc) and binding antibodies were detected by peroxidase-labeled anti-rabbit immunoglobulins antibody (DAKO, P0448). Binding activity is shown by the absorbance of 450–630 nm. ( B ) Diluted purified polyclonal antibodies were incubated with immobilized recombinant mouse CD14-His and binding antibodies were detected by peroxidase-labeled anti-rabbit immunoglobulins antibody (DAKO, P0448). Binding activity is shown by the absorbance of 492 nm.

Article Snippet: Twenty blood samples were centrifuged at 2000 g for 15 min at 4 °C, and plasma fractions were collected and used to determine the levels of presepsin and sCD14 using the developed mouse presepsin kit and the commercially available Quantikine CD14 ELISA kit (R&D systems, Minneapolis, MN, USA).

Techniques: Incubation, Recombinant, Binding Assay, Labeling, Activity Assay, Purification

Journal: Scientific Reports

Article Title: Development of a newly immunoassay specific for mouse presepsin (sCD14-ST)

doi: 10.1038/s41598-022-22096-1

Figure Lengend Snippet: Representative mouse presepsin standard curve with dynamic range 4.7–300 pg/mL of presepsin-Fc protein of the developed ELISA. Each point representative of duplicate measurements.

Article Snippet: Twenty blood samples were centrifuged at 2000 g for 15 min at 4 °C, and plasma fractions were collected and used to determine the levels of presepsin and sCD14 using the developed mouse presepsin kit and the commercially available Quantikine CD14 ELISA kit (R&D systems, Minneapolis, MN, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Development of a newly immunoassay specific for mouse presepsin (sCD14-ST)

doi: 10.1038/s41598-022-22096-1

Figure Lengend Snippet: Linearity of the developed ELISA for mouse presepsin in serial diluted mouse serum. The three individual serum samples were serially diluted as indicated for determination by the developed ELISA. Each point representative the mean value of duplicate measurements.

Article Snippet: Twenty blood samples were centrifuged at 2000 g for 15 min at 4 °C, and plasma fractions were collected and used to determine the levels of presepsin and sCD14 using the developed mouse presepsin kit and the commercially available Quantikine CD14 ELISA kit (R&D systems, Minneapolis, MN, USA).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Development of a newly immunoassay specific for mouse presepsin (sCD14-ST)

doi: 10.1038/s41598-022-22096-1

Figure Lengend Snippet: Reactivity with recombinant mouse CD14-His and mouse presepsin prepared from mouse CD14-His by incubation with cathepsin D. Two antigens were assessed using the developed ELISA. Absorbance is shown by the mean value of duplicate measurements.

Article Snippet: Twenty blood samples were centrifuged at 2000 g for 15 min at 4 °C, and plasma fractions were collected and used to determine the levels of presepsin and sCD14 using the developed mouse presepsin kit and the commercially available Quantikine CD14 ELISA kit (R&D systems, Minneapolis, MN, USA).

Techniques: Recombinant, Incubation, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Development of a newly immunoassay specific for mouse presepsin (sCD14-ST)

doi: 10.1038/s41598-022-22096-1

Figure Lengend Snippet: Structure of mouse presepsin and its amino acid sequences in CD14. ( A ) Presepsin standard is a 68-residue-long single polypeptide having two intramolecular disulfide bonds. CD14 estimated to processed by proteolytic cleavage at the cathepsin D cleavage site (64R–65E) to generate presepsin (sCD14-ST) in mice. ( B ) Amino acid sequences of immunized peptide for generate anti-mouse presepsin antibodies.

Article Snippet: Twenty blood samples were centrifuged at 2000 g for 15 min at 4 °C, and plasma fractions were collected and used to determine the levels of presepsin and sCD14 using the developed mouse presepsin kit and the commercially available Quantikine CD14 ELISA kit (R&D systems, Minneapolis, MN, USA).

Techniques: Residue